Coding

Part:BBa_K4437002:Design

Designed by: Tithi Bhatt   Group: iGEM22_Calgary   (2022-10-12)


NisQ with an N-terminus NusA solubility factor and 6x His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1711
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nisin GB1 (BBa_K4437001) includes, a T7 promoter, a lac operon, RBS, TEV protease site, 6XHis-tag, two enterokinase cut sites, nisQ, and T7 terminator. The two enterokinase sites serves to make sure the 6XHis-tag is cleaved off and surely leaves us with nisQ. The lac operon functions to be able to use different protein induction media such as autoinduction medias when expression protein. The presence of the lac operon will allow the bacteria to switch from a glucose sugar source to a lactose sugar source. The addition of NusA to the sequence allows for better solubility as it serves a function as a solubility tag. The rest if the elements are regulatory elements necessary to express our protein.


Source

It came from a paper.

References